BW1J

94101901, mouse skin, Epithelial

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: BW1J, 94101901
• fonte biológica: mouse skin
• modo de crescimento: Adherent
• cariótipo: Hypotetraploid
• morfologia: Epithelial
• produtos: Alpha-foetoprotein and Albumin
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse hepatoma

Descrição de linhagem celular

A mouse hepatoma isolated from the BW7756 transplantable hepatoma carried in C57 leaden/J mice (see reference: Differentiation). Will synthesise in appreciable amounts only hepatic proteins that appear early in foetal development. The majority of cells contain at least two and often several nucleoli, fat droplets are rare. A mixture of BW1-J and Fao cells were treated with inactive Sendai Virus to produce hybrids at 1 hybrid colony/50 BW1-J cells.

Meio de cultura

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)

Rotina de subcultura

Split semi-confluent cultures (70%) 1:50 i.e. seeding at 2x1000 - 5x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change three times a week.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.