P3U1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse skin
• embalagem: tube of 5 μg 85011417-DNA-5UG; pkg of vial of cells 85011417-1VL
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse myeloma

Descrição de linhagem celular

Derivative of P3X63Ag8, known as the Kearney line, the cell line is a non secretor. HGPRT- and HAT sensitive. It is useful for fusion with spleen cells to produce hybridomas or for hybridisation studies with other myelomas or lymphomas.

Aplicação

Fusion partner, hybridisation and tumourigenicity studies

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Rapid cell death occurs when cell concentration exceeds 1,000,000 cells/ml.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.