M3 Clone M-3

93120827, mouse skin, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: M3 Clone M-3, 93120827
• fonte biológica: mouse skin
• modo de crescimento: Adherent
• cariótipo: Modal no. 83; hypertetraploid
• morfologia: Epithelial
• produtos: Melanin
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse Cloudman S 91 melanoma

Descrição de linhagem celular

Melanin producing cell line, derived from a Cloudman S 91 melanoma induced in a F1 (CxDBA) male mouse. The line is tumourigenic in mice and susceptible to a variety of viruses including herpes simplex, vaccinia, pseudorabies and vesicular stomatitis virus (Indiana virus).

Aplicação

Tumourigenicity studies

Meio de cultura

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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