VERO Cell Line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 84113001, African green monkey kidney, Fibroblast-like

• Sinônimo(s): Vero 81 Cells, VeroCCL81 Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: African green monkey kidney
• descrição: Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• embalagem: tube of 5 μg 84113001-DNA-5UG; pkg of vial of cells 84113001-1VL
• modo de crescimento: Adherent
• cariótipo: 2n = 60, modal no. 58
• morfologia: Fibroblast-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

Vero cells are anchorage-dependent cells and have wide applications in molecular and cell biology research. Vero cells have a pseudo-diploid karyotype and are preferred cell lines for the propagation of various viruses, including poliovirus, rotavirus influenza and rabies virus. These cells are useful hosts for vaccine production.

Origem de linhagem celular

Monkey African Green kidney

Descrição de linhagem celular

Established from the kidney of a normal adult African Green monkey. Susceptible to a wide range of viruses including polio, rubella, arboviruses and reoviruses.

Aplicação

VERO cell lines have been used:

• in the propagation of Chandipura virus (CHPV)
• in the cytotoxicity assay to evaluate the antioxidant and anti-viral activities of Capsicum annuum methanolic extracts
• for the propagation of T.cruzi

Virus studies: polio, rubella, arboviruses and reoviruses

Meio de cultura

(D6546) + 2 mM (G7513) + 10% (F2442).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.