COS-7 Cell Line

from African green monkey kidney, 87021302, SV40 transformed, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: African green monkey kidney
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Monkey African green kidney, SV40 transformed

Descrição de linhagem celular

Derived from CV-1 simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be handled at laboratory containment level 2.

Aplicação

COS-7 Cell Line has been used to:

• test the functional role of ankyrin B in ouabain/Na,K-ATPase/1,4,5-trisphosphate receptor (IP3R) signaling
• study the expression of green fluorescent protein-tagged constructs of neuropathy target esterase (NTE) and mutant proteins lacking the transmembrane, catalytic and regulatory domains
• study the distribution of peroxisomes

Virus replication studies: SV40

Meio de cultura

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)..

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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