CHO-K1 Cell Line from hamster

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85051005, hamster ovary, Epithelial

• Sinônimo(s): CHO cell clone K1 Cells, CHOK1 Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: hamster ovary
• embalagem: tube of 5 μg 85051005-DNA-5UG; pkg of vial of cells 85051005-1VL
• modo de crescimento: Adherent
• cariótipo: Hypodiploid, modal no. 20
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Hamster Chinese ovary

Descrição de linhagem celular

A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.

Aplicação

CHO-K1 cell line from hamster has been used to study the effect of plasmid pCM01 induction on the association of bacteria with CHO-K1 cells by the gentamicin protection assay. It has also been cultured with or without lactate to measure time-series for cell proliferation.

Nutritional and gene expression studies

Meio de cultura

Ham′s F12+ 2mM Glutamine+ 10% Foetal Bovine Serum FBS / FCS.

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25%Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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