KARPAS 422

06101702, human lymph (lymphoma). Round polygonal singlets or small clusters in suspension

• Sinônimo(s): KARPAS, 422
• Código UNSPSC: 41106514

Propriedades

• product name: KARPAS 422, from human lymph(lymphoma), 06101702
• fonte biológica: human lymph (lymphoma)
• modo de crescimento: Suspension
• cariótipo: Hyperdiploid with 10% polyploidy
• morfologia: Round polygonal cells growing singly or in small clusters in suspension
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice

Descrição

Origem de linhagem celular

Human B cell non-Hodgkin lymphoma

Descrição de linhagem celular

Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).

Perfil do DNA

Not specified

Meio de cultura

RPMI 1640 + 2 mM Glutamine + 20% Fetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 0.5 - 2 x 1000,000 cells/ml; 5% CO₂; 37 °C. Doubling time approximately 60-90 hours. Split saturated culture 1:2 every 2-4 days. Round polygonal cells growing singly or in small clusters in suspension.

Outras notas

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Exoneração de responsabilidade

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.