Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

More Documents

N4888

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Ham’s F-12

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Nutrient Mixture F-12 is suitable for the growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using Chinese hamster ovary (CHO) cells.

Application

Nutrient Mixture F-12 Ham has been used:
  • in Minimum Essential Medium to maintain SH-SY5Y human neuroblastoma cells
  • to culture female-originated neuroblastoma cells
  • to culture primary retinal pigment epithelium (RPE) cells and RPE cell lines

Reconstitution

Supplement with 0.146 g/L L-glutamine.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Kambiz Shekdar et al.
Biotechnology letters, 43(5), 949-958 (2021-03-09)
Chromovert® Technology is presented as a new cell engineering technology to detect and purify living cells based on gene expression. The technology utilizes fluorogenic oligonucleotide signaling probes and flow cytometry to detect and isolate individual living cells expressing one or
Roger Oria et al.
Nature, 552(7684), 219-224 (2017-12-07)
Cells can sense the density and distribution of extracellular matrix (ECM) molecules by means of individual integrin proteins and larger, integrin-containing adhesion complexes within the cell membrane. This spatial sensing drives cellular activity in a variety of normal and pathological
Yinan Jiang et al.
Molecular immunology, 82, 123-136 (2017-01-11)
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is known as a key molecule to induce cancer cell apoptosis, has also been found to participate in the process of ischemia/reperfusion (I/R) injury. Infiltrated macrophages play dual roles in inflammatory injury and
Chanan Meydan et al.
Frontiers in immunology, 11, 590870-590870 (2020-11-10)
The COVID-19 pandemic exerts inflammation-related parasympathetic complications and post-infection manifestations with major inter-individual variability. To seek the corresponding transcriptomic origins for the impact of COVID-19 infection and its aftermath consequences, we sought the relevance of long and short non-coding RNAs
Mustafa Orhan Puskullu et al.
Bioorganic chemistry, 101, 104014-104014 (2020-07-01)
In this study, a total of 22 piece quinoline-3-carbaldehyde hydrazone derivative compounds were designed and synthesized, 2 of which were not original, their antimicrobial activities were determined with microdilution method and their in vitro cytotoxic effect was investigated in MCF-7
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service