P3X63Ag8U.1

94120802, mouse blood, Lymphoblast

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: P3X63Ag8U.1, 94120802
• fonte biológica: mouse blood
• embalagem: tube of 5 μg 94120802-DNA-5UG; pkg of vial of cells 94120802-1VL
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse Myeloma

Descrição de linhagem celular

P3x63Ag8U.1 is a derivative of the P3x63Ag8 cell line (Sigma Catalogue number. 85011401) from the mouse myeloma MOPC21, which was derived from a female BALB/c mouse. This variant no longer synthesises gamma-1 chains but still produces kappa light chains which are not secreted.

Aplicação

This cell line is useful for fusion to spleen cells to produce hybridomas or for hybridisation studies with other myelomas or lymphomas. Can also be used for immunoglobulin production.

Meio de cultura

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures at 5-9x100,000 cells/ml; 5% CO₂; 37°C.

Outras notas

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