SP2/O-Ag14 Cell Line murine

Non-producing hybridoma, 85072401

• Sinônimo(s): Mouse Cell Line
• Código UNSPSC: 12352207

Propriedades

• fonte biológica: mouse spleen
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Not specified
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse x Mouse myeloma, non-producing

Descrição de linhagem celular

Sp2/0-Ag14 is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media. HAT sensitive mouse myeloma hybrid suitable for use as a hybridoma fusion partner - in this sense, functionally it is a myeloma cell line and often referred to as such in the literature.

Aplicação

Fusion partner

Meio de cultura

RPMI 1640 or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. HAT sensitive.

Outras notas

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