3T3 clone A31 Cell Line from mouse

86110401, mouse embryo, Fibroblast

• Sinônimo(s): 3T3-A31 Cells, BALB/3T3 clone A31 Cells, BALB/c 3T3 clone A31 Cells
• Código UNSPSC: 41106514

Propriedades

• product name: 3T3 clone A31 Cell Line from mouse, 86110401
• fonte biológica: mouse embryo
• descrição: Mouse BALB/c embryo
• modo de crescimento: Adherent
• cariótipo: 2n = 40, hypotetraploid
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse BALB/c embryo

Descrição de linhagem celular

Cells have now lost their contact inhibition.

Aplicação

3T3 clone A31 cell line from mouse has been used:

• to study in vitro cytotoxicity and genotoxicity of retinyl palmitate–loaded poly(lactide-co-glycolide) nanoparticles for the topical treatment of skin diseases
• for the characterization of the phototoxicity of Vemurafenib, which is a B-Raf serine/threonine kinase inhibitor
• for the phototoxicity assessment of systemically applied compounds and drug candidates

Tumourigenicity and virus studies: SV40 and MSV

Meio de cultura

DMEM + 2mM Glutamine + 5% New Born Calf Serum (NBCS) + 5% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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