A431 Cell Line human

85090402, human skin, Epithelial

• Sinônimo(s): A-431 Cells, A431/P Cells
• Código UNSPSC: 41106514

Propriedades

• Nome do produto: A431 Cell Line human, squamous carcinoma, 85090402
• fonte biológica: human skin
• modo de crescimento: Adherent
• cariótipo: Hypertriploid
• morfologia: Epithelial
• produtos: Not specified
• receptores: Epidermal growth factor (EGF)
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human squamous carcinoma

Descrição de linhagem celular

Derived from an epidermal carcinoma of the vulva taken from an 85 yr old female. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.

Aplicação

A431 Cell Line human has been used:

• to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
• to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
• to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants

Cell cycle studies

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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