CHSE-214 Cell Line from salmon

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91041114, fish embryo, Not specified

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: fish embryo
• embalagem: tube of 5 μg 91041114-DNA-5UG; pkg of vial of cells 91041114-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Not specified
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Salmon embryo

Descrição de linhagem celular

Derived from a Chinook salmon (Oncorhynchus tshawytscha) embryo, susceptible to a wide range of fish viruses and in many instances replicate high titres.

Aplicação

CHSE-214 cell line has been used for the growth and titration of infectious pancreatic necrosis virus (IPNV) from samples. It has also been used to carry out cell infection assays to determine the effect of tropodithietic acid (TDA)-exposed isolates.

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e seeding at 1-4 x 10,000 cells per cm using 0.25% trypsin or trypsin/EDTA, cells detach after 5-10 minutes at room temperature. Grow cells in dark (e.g. wrapped in foil); 5% CO₂; 21°C. Freeze cells in 5% DMSO and 95% foetal bovine serum (FBS). Fish cell lines detach easily during transit if the culture is too young. For resuscitation seed flasks at 4-5 x 10,000 cells per cm and then split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.