FHM

88102401, fish colon (posterior), Epithelial-like

• Código UNSPSC: 41106514

Propriedades

• product name: FHM, 88102401
• fonte biológica: fish colon (posterior)
• modo de crescimento: Adherent
• cariótipo: Modal no. 51
• morfologia: Epithelial-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Fish Fat Head minnow

Descrição de linhagem celular

Derived from tissue posterior to the anus of normal adult minnows. Reported to be capable of growth over a wide temperature range from 4°C-36°C with maximum growth at 34°C. Support growth of the virus of infectious pancreatic necrosis (IPN), cat fish tumour virus (CTV) and Granoff frog virus (FV1). ECHO-II will multiply in FHM but the cytopathic effect is not extensive.

Aplicação

Virus studies: Inf. pacr. necrotic virus, cat fish tumour, and Granoff′s frog viruses

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:3; 34°C. Dislodge cells by agitation and scraping. Cells are reported to multiply over a wide range of temperatures 0°-36°C, with maximum growth at about 34°C. It is recommended that cultures are grown at 34

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