SW480 Cell Line human

87092801, human colon, Epithelial

• Sinônimo(s): SW-480 Cells, SW480E Cells
• Código UNSPSC: 41106514

Propriedades

• product name: SW480 Cell Line human, 87092801
• fonte biológica: human colon
• descrição: Human colon adenocarcinoma
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human colon adenocarcinoma

Descrição de linhagem celular

Derived from a grade 3-4 colon adenocarcinoma. The initial cultures contained a mixture of epithelial and bipolar cells, but subsequently epithelial cells predominated. The cells produce CEA.

Aplicação

Human SW480 cell line has been used to study the antiproliferative effects of F. mughlea Pesmen on cancer cells. It has also been used as an in vitro model to study key cellular processes required for metastasis. The cell line is used to study the effects of the transforming growth factor-β (TGF-β) pathway and heat shock protein 90 (Hsp90) on the growth of cells.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 12
D16S539: 13
D5S818: 13
D7S820: 8
THO1: 8
TPOX: 11
vWA: 16

Meio de cultura

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin; 37°C. Medium change every 4-5 days. CO₂ is not required.

Outras notas

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