HCT-15

91030712, human colon, Epithelial-like

• Código UNSPSC: 41106514

Propriedades

• product name: HCT-15, 91030712
• fonte biológica: human colon
• modo de crescimento: Adherent
• cariótipo: 2n = 46, quasidiploid
• morfologia: Epithelial-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human colon adenocarcinoma

Descrição de linhagem celular

Colorectal adenocarcinoma which is tumorigenic in nude mice.The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.

Aplicação

HCT-15 cell line has been used to study the role and efficiency of nexrutine (NX), an extract from Phellodendron amurense, against colon cancer.

Meio de cultura

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS) (heat inactivated).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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