Sf9 Cell Line from Spodoptera frugiperda pupal ovarian tissue

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89070101, insect ovary, Spherical

• Sinônimo(s): SF-9 Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: insect ovary
• embalagem: tube of 5 μg 89070101-DNA-5UG; pkg of vial of cells 89070101-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Spherical
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Spodoptera frugiperda pupal ovarian tissue

Descrição de linhagem celular

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems.

Aplicação

Sf9 cell line has been used for Baculovirus viral studies. It has also been used for in vitro kynurenine-3-monooxygenase (KMO) inhibition assay.

Meio de cultura

TC100 + 2mM Glutamine. (The medium can be supplemented with 10% Foetal Bovine Serum (FBS) if required).

Rotina de subcultura

Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 9% DMSO/91% FBS. Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.

Outras notas

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