CHSE-214
21714, fish embryo, Fibroblast-like
Sinônimo(s):
CHSE/F
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About This Item
Código UNSPSC:
41106514
fonte biológica:
fish embryo
modo de crescimento:
Adherent
cariótipo:
Not specified
morfologia:
Fibroblast-like
produtos:
Not specified
receptores:
Not specified
Produtos recomendados
Nome do produto
CHSE-214, 00021714
fonte biológica
fish embryo
modo de crescimento
Adherent
cariótipo
Not specified
morfologia
Fibroblast-like
produtos
Not specified
receptores
Not specified
técnica(s)
cell culture | mammalian: suitable
Condições de expedição
dry ice
temperatura de armazenamento
−196°C
Categorias relacionadas
Origem de linhagem celular
Common bluegill (Lepomis macrochirus), normal
Descrição de linhagem celular
The fish line CHSE/F was derived from Common bluegill (Lepomis macrochirus) embryo. It is susceptible to various fish viruses including infectious hematopoietic necrosis virus (IHNV); viral haemorrhagic septicaemia virus (VHSV); spring viraemia of carp virus (SVCV); and infectious pancreatic necrosis virus (IPNV).
ECACC has been advised by the CCLV that this cell line is derived from Lepomis macrochirus (Common bluegill) not Oncorhynchus tshawytscha (Chinook salmon) as originally reported. This has been confirmed by DNA sequencing at ECACC. The CCLV have changed the cell line name to CHSE/F to indicate this. The line is used in viral diagnostics and gives better results than BF-2 (Sigma Catalogue number 00021712), a commonly used cell line of Lepomis macrochirus. The sequence of both lines (BF-2 and CHSE/F possess the same nucleotide exchange but are different in culture behaviour and in virus propagation.
ECACC has been advised by the CCLV that this cell line is derived from Lepomis macrochirus (Common bluegill) not Oncorhynchus tshawytscha (Chinook salmon) as originally reported. This has been confirmed by DNA sequencing at ECACC. The CCLV have changed the cell line name to CHSE/F to indicate this. The line is used in viral diagnostics and gives better results than BF-2 (Sigma Catalogue number 00021712), a commonly used cell line of Lepomis macrochirus. The sequence of both lines (BF-2 and CHSE/F possess the same nucleotide exchange but are different in culture behaviour and in virus propagation.
Aplicação
Fish virus studies
Meio de cultura
EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 120mg/l Sodium Pyruvate(NaP) (S8636) + 10% FBS / FCS (F2442).
Rotina de subcultura
Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; grow cells in dark (e.g. wrapped in foil) at 20-26ºC with 26ºC optimum temperature; monolayer is stable for at least 10 days with maintenance medium containing 1-2% FBS.
Outras notas
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