BF-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 21712, fish caudal trunk, Fibroblast-like

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: fish caudal trunk
• embalagem: tube of 5 μg 00021712-DNA-5UG; pkg of vial of cells 00021712-1VL
• modo de crescimento: Adherent
• cariótipo: 2n=48, stem-line = 48 with scattering from 36-121
• morfologia: Fibroblast-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Fish, bluegill fry

Descrição de linhagem celular

The cell line BF-2 has been derived from a trypsinised suspension of pooled caudal portions of the trunk of 1 year old fingerlings of Bluegill fry (Lepomis macrochirus). Cells have been reported to be susceptible to a variety of fish viruses including lymphocystis virus, viral haemorrhagic septicaemia virus (VHSV), infectious pancreatic necrosis virus (IPNV) and tadpole edema virus (TEV) of frogs. The cell monolayer is confluent after 48-56 hours using a 1:4 split ratio. Catalogue no. at CCLV (depositor) is RIE 193.

Meio de cultura

EMEM(EBSS) (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 120 mg/l Sodium Pyruvate(NaP) (S8636) + 10% FBS / FCS (F2442).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; cells replicate at 15-33ºC, optimal temperature 23-26ºC; monolayer is stable for at least 10 days, maintenance medium should contain MEM(EBSS) + 740 mg/l NaHCO₃ + 1.2g/l

Outras notas

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