MDCK

85011435, dog kidney, Epithelial-like

• Sinônimo(s): MDCK1 Cells, MDCKI Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• Código UNSPSC: 41106514
• eCl@ss: 32190102

Propriedades

• product name: MDCK, 85011435
• fonte biológica: dog kidney
• modo de crescimento: Adherent
• cariótipo: 2n = 78
• morfologia: Epithelial-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Canine Cocker Spaniel kidney

Descrição de linhagem celular

Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2).

Aplicação

Virus studies: SVEV, VSV, Vaccinia, reoviruses and inf. canine hepatitis virus

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

Outras notas

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