MDCK Cell Line canine

84121903, canine kidney, Epithelial

• Sinônimo(s): MDCK (NBL-2) Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• Código UNSPSC: 41106514
• eCl@ss: 32190102

Propriedades

• product name: MDCK Cell Line canine, 84121903
• fonte biológica: canine kidney
• forma: cell suspension
• modo de crescimento: Adherent
• cariótipo: 2n = 78
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Canine Cocker Spaniel kidney

Descrição de linhagem celular

From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.

Aplicação

Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

Outras notas

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