MDCK-SIAT1 Cell Line canine

5071502, canine kidney, Epithelial

• Sinônimo(s): MDCK-SIAT1 Cells
• Código UNSPSC: 41106514

Propriedades

• product name: MDCK-SIAT1 Cell Line canine, 05071502
• fonte biológica: canine kidney
• descrição: Canine Cocker Spaniel Kidney Sialic Acid Over Expression
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Not specified
• receptores: Enhanced expression of 6-linked sialic acids
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Canine Cocker Spaniel Kidney Sialic Acid Over Expression

Descrição de linhagem celular

This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold?higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-α2,6-galactose moieties.

Meio de cultura

DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

Outras notas

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