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R4655

Sigma-Aldrich

Anti-Rab27 (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinônimo(s):

Anti-RAB27A, member RAS oncogene family

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

rabbit

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

buffered aqueous solution

peso molecular

antigen ~25 kDa

reatividade de espécies

human, rat

concentração

~1.5 mg/mL

técnica(s)

indirect immunofluorescence: 5-10 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin
western blot: 2-5 μg/mL using whole extract of human A431 cells

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... RAB27A(5873)
rat ... Rab27a(50645)

Descrição geral

Rab27 (Ras-related protein) is a member of the Rab family of small guanosine triphosphatases (GTPases). Rab27 is widely expressed in non-neuronal regulated secretory cells, including exocrine, endocrine, ovarian, and hematopoietic cells.
Rab27A (Ras-related protein) belongs to the Rab family of small GTPase proteins. It is located on chromosome 15q21.

Imunogênio

synthetic peptide corresponding to amino acid residues 45-57 of human RAB27A, with an added cysteine, conjugated to KLH. The corresponding sequence differs by one amino acid in rat and mouse Rab27A and by 2 amino acids in human RAB27B.

Aplicação

Anti-Rab27 (N-terminal) antibody has been used in immunoblotting, immunofluorescence.

Ações bioquímicas/fisiológicas

In humans, Rab27A (Ras-related protein) plays important roles in the development of cancers. It is considered as a prognostic biomarker for CRC (colorectal cancer) patients. It may participate in the transfer of protein and signal transduction mediated by GTPase. Rab27 mutation is linked with Griscelli syndrome type 2. It is also responsible for the innate immune resistance against invading microorganisms.
Rab27 (Ras-related protein) regulates the exocytosis of these cell-specific store organelles, using multiple organelle-specific effector proteins, such as granuphilin and melanophilin.

forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Hironori Higashio et al.
Scientific reports, 6, 22539-22539 (2016-03-05)
Mast cell degranulation is regulated by the small guanosine triphosphatases (GTPases) Rab27a and Rab27b, which have distinct and opposing roles: Rab27b acts as a positive regulator through its effector protein Munc13-4, a non-neuronal isoform of the vesicle-priming Munc13 family of
Rab27 effectors, pleiotropic regulators in secretory pathways
Fukuda M, et al.
Traffic, 14(9), 949-963 (2013)
A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport
Strom M, et al.
The Journal of Biological Chemistry (2002)
Rab27a was identified as a prognostic biomaker by mRNA profiling, correlated with malignant progression and subtype preference in gliomas
Wang H, et al.
PLoS ONE, 9(2) (2014)
Vectorial release of hepatitis E virus in polarized human hepatocytes
Capelli N, et al.
Journal of virology, 93(4), e01207-e01218 (2019)

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