AtT20

87021902, mouse brain, Small round cells growing as large aggregates

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: AtT20, 87021902
• fonte biológica: mouse brain
• modo de crescimento: Aggregates in suspension
• cariótipo: Diploid
• morfologia: Small round cells growing as large aggregates
• produtos: Adrenocorticotropic hormone (ACTH)
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse LAF1 pituitary gland tumour

Descrição de linhagem celular

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

Aplicação

Hormone studies

Meio de cultura

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

Rotina de subcultura

Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi

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