AtT-20/D16v-F2

94050406, mouse brain, Epithelial

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: AtT-20/D16v-F2, 94050406
• fonte biológica: mouse brain
• embalagem: tube of 5 μg 94050406-DNA-5UG; pkg of vial of cells 94050406-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse pituitary tumour

Descrição de linhagem celular

This F2 subclone was developed from original AtT-20, an andrenocorticotropic hormone (ACTH) secreting cell line established from murine pituitary tumour. These cells are readily transfected using a standard calcium phosphate protocol.

Aplicação

AtT-20/D16v-F2 has been used to study the role of the G-protein Rasd1 in hypothalamic response to neuronal activation. It has also been used to study the effect of cAMP and glucocorticoid treatment on cAMP-responsive element binding protein 3 like 1 (CREB3L1).

Meio de cultura

DMEM + 2mM Glutamine + 10% FBS.

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:5 using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cell clumps must be broken up to prevent cells growing in suspension.

Outras notas

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