b.End3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96091929, mouse brain, Endothelial-like

• Sinônimo(s): bEND.3 Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse brain
• embalagem: tube of 5 μg 96091929-DNA-5UG; pkg of vial of cells 96091929-1VL
• disponibilidade: available only in EU
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Endothelial-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse SV129 brain endothelioma

Descrição de linhagem celular

The cell line b.End3 has been established from brain endothelial cells of SV129 mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End3 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as ICAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.

Aplicação

Study of endothelial cell growth, differentiation and influence of oncogenes.

Meio de cultura

DMEM + 2mM Glutamine + 5μM 2-Mercaptoethanol (2ME) + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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