BC3H1

86093001, mouse brain, Fibroblast

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: BC3H1, 86093001
• fonte biológica: mouse brain
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast
• produtos: Acetylcholine
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse brain tumour

Descrição de linhagem celular

Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea, possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.

Aplicação

Acetylcholine receptor studies.

Meio de cultura

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Rotina de subcultura

Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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