MCF7 Cell Line human

86012803, human breast (adenocarcinoma), Epithelial-like

• Sinônimo(s): IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells, ssMCF-7 Cells
• Código UNSPSC: 41106514
• eCl@ss: 32190102

Propriedades

• Nome do produto: MCF7 Cell Line human, from human breast(adenocarcinoma), 86012803
• fonte biológica: human breast (adenocarcinoma)
• modo de crescimento: Adherent
• cariótipo: 2n = 46, hypertriploid to hypotetraploid
• morfologia: Epithelial-like
• produtos: Not specified
• receptores: Reported to express the oestrogen and progesterone receptor
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian breast adenocarcinoma

Descrição de linhagem celular

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

Aplicação

Tumourigenicity and virus studies: Type B and C viruses

Perfil do DNA

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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