LNCap Clone FGC Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89110211, human prostate (carcinoma), Epithelial-like

• Sinônimo(s): LNCaP-FGC Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human prostate (carcinoma)
• embalagem: tube of 5 μg 89110211-DNA-5UG; pkg of vial of cells 89110211-1VL
• modo de crescimento: Adherent
• cariótipo: Pseudodiploid male; seven marker chromosomes, modal number 46, range 33 to 91
• morfologia: Epithelial-like
• produtos: Prostate acid phosphatase, prostate specific antigen
• receptores: Androgen, estrogen
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): metastasis
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian prostate carcinoma

Descrição de linhagem celular

Derived from a metastasis at the left supraclavicular lymph node of a 50 year old patient with a confirmed diagnosis of metastatic prostate carcinoma. Growth and acid phosphatase production is affected by 5-alpha-dihydrotestosterone. They do not form a uniform monolayer and attach only lightly to the substrate. When shipped, cells detach from flask and can either be incubated 24-48 hours to allow attachment or be collected by centrifugation (150xg, 15 minutes) and reseeded.

Aplicação

LNCap Clone FGC Cell Line has been used:

• to study the effect of sex steroid hormones in regulating the expression of oligoadenylate synthetase 1 (OAS1)
• to study the role of curcumin and resveratrol in redox control and ability to promote apoptosis
• to investigate low molecular weight thiols present in the deproteinized extract of the cell line after derivatization

Meio de cultura

RPMI 1640 + 2mM Glutamine + 1.0 mM sodium pyruvate + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.i.e. seeding at 1-3 x 10,000 cells/cm² Cells grow slowly in clusters and can be disaggregated by repeated pipetting. After subculture cells may take up to 48 hours to reattach. Do not disturb during this period. Medium is rapidly acidified, therefore change twice per week.

Outras notas

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