VCaP Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 6020201, human prostate (metastasis), Epithelial

• Sinônimo(s): Human VCaP Cells, VCaP Cell Culture
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human prostate (metastasis)
• embalagem: tube of 5 μg 06020201-DNA-5UG; pkg of vial of cells 06020201-1VL
• modo de crescimento: Adherent
• cariótipo: Refer to reference (Korenchuk et al., 2001)
• morfologia: Epithelial
• produtos: Not specified
• receptores: Androgen receptor
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): metastasis
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Prostate Cancer Metastasis

Descrição de linhagem celular

This Vertebral-Cancer of the Prostate (VCaP) cell line was established from prostate cancer tissue harvested from a metastatic lesion to a lumbar vertebral body of a patient with hormone refractory prostate cancer. The cells are reported to express large quantities of prostate specific antigen (PSA). This cell line also expresses prostatic acid phosphatase (PAP), cytokeratin-18 and the androgen receptor, and is androgen sensitive in vitro and in vivo. This cell line offers a cell-based model system of human prostate cancer. The cells are tumourigenic in SCID mice.

Perfil do DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19

Meio de cultura

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Pellet cells by centrifugation and remove trypsin after inactivation of trypsin at each subculture.

Outras notas

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