T47D Cell Line human

85102201, human breast, Epithelial

• Sinônimo(s): T-47-D Cells, T-47D Cells, T47D:A Cells
• Código UNSPSC: 41106514

Propriedades

• product name: T47D Cell Line human, 85102201
• fonte biológica: human breast
• descrição: Human breast tumor
• modo de crescimento: Adherent
• cariótipo: 2n = 46, hypertriploid, modal no. 65
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human breast tumour

Descrição de linhagem celular

Established from the pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The cells carry receptors for a variety of steroids.

Aplicação

T47D cell line human has been used:

• to study the effects of a polysaccharide–protein complex (PPC) isolated from metabolites of gut bacteria Raoultella ornithinolytica from the earthworm Dendrobaena veneta against Candida albicans
• has been cultured and incubated with bone marrow-derived macrophages to study their migration and podosome formation
• to study the anti-proliferative effects of two novel classes of potential antimetabolites on T47D cells

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14

Meio de cultura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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