Ishikawa Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 99040201, human uterus, Epithelial-like

• Sinônimo(s): ISHI Cells, Ishikawa Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human uterus
• embalagem: tube of 5 μg 99040201-DNA-5UG; pkg of vial of cells 99040201-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial-like
• produtos: Not specified
• receptores: Oestrogen, progesterone
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Asian endometrial adenocarcinoma

Descrição de linhagem celular

The cell line Ishikawa was established from an endometrial adenocarcinoma from a 39 year old woman. The cells induced well differentiated adenocarcinoma in athymic nude mice. Estrogen and progesterone receptors were demonstrated both in cell culture and in induced tumours. Ishikawa has been reported to produce corticotropin-releasing hormone, placental alkaline phosphatase and chorionic gonadotrophin, and responded to steroid hormones. The cell line has been eradicated from mycoplasma at ECACC using BM-cyclin. Ishikawa was temporarily removed from the web site whilst an authentication issue was investigated. Following collaboration with key investigators in this field, ECACC is confident that this cell line is Ishikawa.

Aplicação

Ishikawa Cell Line human has been used to study the effect of 1,25-Dihydroxy-Vitamin D3 (1,25(OH)2D3) on cytosolic pH regulation and glycolytic flux. It has also been used to study endometrial adenocarcinoma.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,12
D16S539: 9
D5S818: 10,11
D7S820: 9,10
THO1: 9,10
TPOX: 8
vWA: 14,17

Meio de cultura

MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC; cells tend to pile up.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.