MFE-280

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 98050131, human endometrium, Epithelial

• Sinônimo(s): MFE280 Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human endometrium
• embalagem: tube of 5 μg 98050131-DNA-5UG; pkg of vial of cells 98050131-1VL
• modo de crescimento: Adherent
• cariótipo: Near diploid
• morfologia: Epithelial
• produtos: Placental protein 14
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human caucasian endometrial adenocarcinoma

Descrição de linhagem celular

MFE-280 was derived from a recurrent, poorly differentiated endometrial carcinoma from a 78 year old patient. Initially, cells were grown as aggregates in suspension for one year, after which they were transferred to monolayer culture. The karyotype analysis revealed near-diploidy with a complex heterogeneous aberration pattern. MFE-280 were found to be positive for cytokines 7, 8, 18 and 19 as well as for vimentin. In addition, the cells express the placental protein 14, PP14. They have been shown to be tumorigenic in nude mice.

Aplicação

Tumour biology studies

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 10,12
D16S539: 12
D5S818: 11,12
D7S820: 10
THO1: 7
TPOX: 9,10
vWA: 17

Meio de cultura

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding 2-4 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Outras notas

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