MFE-296

98031101, human endometrium, Epithelial

• Sinônimo(s): MFE296 Cells
• Código UNSPSC: 41106514

Propriedades

• product name: MFE-296, 98031101
• fonte biológica: human endometrium
• modo de crescimento: Adherent
• cariótipo: Near Tetraploid
• morfologia: Epithelial
• produtos: Not specified
• receptores: Androgen receptor
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human caucasian endometrial adenocarcinoma

Descrição de linhagem celular

MFE-296 was obtained from a moderately differentiated human endometrial adenocarcinoma. The cells were shown to express functionally intact androgen receptors. Proliferation was inhibted by progestin R5020 and dihydrotestosterone (DHT), the latter one being antagonised by the antiandrogen Casodex. MFE-296 express vimentin and the cytokeratins 7, 8, 18 and 19. Karyotype analysis revealed near tetraploidy for most cells. Further more, the cells have been shown to be tumorigenic in nude mice.

Aplicação

Tumour biology studies, receptor analysis

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,13
D16S539: 11,14
D5S818: 11,13
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 14,18

Meio de cultura

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding 1-2 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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