GP5d

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95090715, human colon, Epithelial

• Sinônimo(s): GP5d Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human colon
• embalagem: tube of 5 μg 95090715-DNA-5UG; pkg of vial of cells 95090715-1VL
• modo de crescimento: Adherent
• cariótipo: Diploid
• morfologia: Epithelial
• produtos: Not specified
• receptores: Epidermal growth factor (EGF)
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian colon adenocarcinoma

Descrição de linhagem celular

GP5d has been established from the same adenocarcinoma as GP2d (Sigma Catalogue number. 95090714). This has been confirmed by STR profiling at ECACC. The cells were derived from a local recurrence of Duke′s grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radio therapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess changes consistent with the usual pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP5d cells express relatively more TGF α mRNA than GP2d cells. GP5d cells undergo a marked epithelial-mesemchymal transition when treated with EGF.

Aplicação

Colon tumour assays, influence of growth factors and adhesion molecules on colonic tumour cells

Meio de cultura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 5x10,000 to 1x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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