COLO 205 Cell Line human

87061208, human colon. Round, refractile cells in suspension. Some cuboidal morphology in monolayer.

• Sinônimo(s): COLO205 Cells
• Código UNSPSC: 41106514

Propriedades

• product name: COLO 205 Cell Line human, 87061208
• fonte biológica: human colon
• descrição: Human Caucasian colon adenocarcinoma
• modo de crescimento: Suspension
• cariótipo: Hypertriploid modal no 71-78
• morfologia: Round and refractile cells in suspension. Some cuboidal cells in the monolayer
• produtos: Carcinoembryonic antigen (CEA)
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

Isolated from ascitic fluid of a 70 year old Caucasian male with carcinoma of the colon. Produces carcinoembryonic antigen.

Origem de linhagem celular

Human Caucasian colon adenocarcinoma

Descrição de linhagem celular

COLO 205, COLO 201 (Sigma product no. 87091201) and COLO 206 (Sigma product no. 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 205 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 205 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer.

Aplicação

tumourigenic in suitable animals

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain suspension cells at 3-9x10⁵ cells/cm³. Split subconfluent cultures of attached cells 1:3 to 1:6 seeding at 2-3x10⁴ cells/cm²; 5% CO₂; 37°C.

Outras notas

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