SK-OV-3 Cell Line human

91091004, human ovary, Epithelial

• Sinônimo(s): SKOV-3 Cells, SKOV3 Cells
• Código UNSPSC: 41106514

Propriedades

• product name: SK-OV-3 Cell Line human, 91091004
• fonte biológica: human ovary
• descrição: Human Caucasian ovary adenocarcinoma
• modo de crescimento: Adherent
• cariótipo: Hypodiploid to hypotetraploid
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian ovary adenocarcinoma

Descrição de linhagem celular

Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells.

Aplicação

SK-OV-3 cell line from humans has been used to study chimeric adenoviruses of Ad5 (species C) displaying proteins of the naturally enteric Ad40 (species F) as vectors for gene delivery.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 12
D5S818: 11
D7S820: 13,14
THO1: 9,9.3
TPOX: 8,11
vWA: 17,18

Meio de cultura

McCoy′s 5a + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-6x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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