MES-SA

95051030, human uterus, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: MES-SA, 95051030
• fonte biológica: human uterus
• modo de crescimento: Adherent
• cariótipo: Modal no. 45, monosomic forms of chromosomes 5, 6 and 7
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human uterus sarcoma

Descrição de linhagem celular

A human uterine sarcoma derived in 1980 from a tumour obtained at the time of a hysterectomy from a 56 year old Caucasian female. The cells form tumours in athymic nude mice, readily form colonies in soft agar and have a reported doubling time of 22-24 hours. The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin. They are resistant to vinblastine dacarbazine, cisplatin, melphalan, vincristine methotrexate and etoposide. The multi drug-resistant cell line MES-SA/Dx5 was established from MES-SA cells grown in the presence of doxorubicin.

Aplicação

Tumourigenicity and chemotherapeutic agent studies

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 11,12
D5S818: 13
D7S820: 7,11
THO1: 6
TPOX: 8,11
vWA: 18

Meio de cultura

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-4 x 10,000 cells/cm² using 0.03% EDTA; 5% CO₂; 37°C. Do not use trypsin.

Outras notas

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