BHK21-InVitrus

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: hamster kidney
• embalagem: tube of 5 μg 05062302-DNA-5UG; pkg of vial of cells 05062302-1VL
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Hamster Syrian kidney, serum-free

Descrição de linhagem celular

BHK21CL13-2P cell line (Sigma Catalogue number. 84111301) adapted to grow in the chemically defined protein- and peptide-free InVitrus^™ medium.

Meio de cultura

InVitrus^™ medium + 4mM Glutamine + 5 ug/ml Insulin. When freezing cells down use InVitrus medium + 4mM Glutamine (50:50 conditioned medium:fresh medium) + 10% DMSO.

Rotina de subcultura

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Informações legais

InVitrus is a trademark of Cell Culture Technologies