C2C12 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91031101, mouse muscle, Myoblast

• Sinônimo(s): C2-C12 Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse muscle
• embalagem: tube of 5 μg 91031101-DNA-5UG; pkg of vial of cells 91031101-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Myoblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse C3H muscle myoblast

Descrição de linhagem celular

Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.

Aplicação

C2C12 Cell Line has been used to study the the expression of OXT and OXTR (oxytocin and its receptor) genes and proteins and in differentiation studies.

Meio de cultura

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum FBS / FCS.

Rotina de subcultura

Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Do not allow cells to reach confluence.

Outras notas

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