C2C12 Cell Line from mouse

91031101, mouse muscle, Myoblast

• Sinônimo(s): C2-C12 Cells
• Código UNSPSC: 41106514

Propriedades

• product name: C2C12 Cell Line from mouse, C3H muscle myoblast, 91031101
• fonte biológica: mouse muscle
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Myoblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse C3H muscle myoblast

Descrição de linhagem celular

Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.

Aplicação

C2C12 Cell Line has been used to study the the expression of OXT and OXTR (oxytocin and its receptor) genes and proteins and in differentiation studies.

Meio de cultura

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum FBS / FCS.

Rotina de subcultura

Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Do not allow cells to reach confluence.

Outras notas

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