Huh-7D 12 Cell Line human

1042712, human liver, Epithelial

• Sinônimo(s): Huh7 Cells, JTC-39 Cells, Japanese Tissue Culture-39 Cells
• Código UNSPSC: 41106514

Propriedades

• Nome do produto: Huh-7D 12 Cell Line human, 01042712
• fonte biológica: human liver
• modo de crescimento: Adherent
• cariótipo: That of original Huh-7
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.

Origem de linhagem celular

Human hepatocellular carcinoma

Descrição de linhagem celular

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

Aplicação

Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18

Meio de cultura

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 C. Media change twice a week

Outras notas

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