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C9658

Sigma-Aldrich

Carboxypeptidase G from Pseudomonas sp.

lyophilized powder, ≥3 units/mg protein

Sinônimo(s):

γ-Glutamyl hydrolase

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About This Item

Número CAS:
9074-87-7
Número da licença da enzima:
3.4.17.11 (BRENDA, IUBMB)
Número CE:
232-978-1
Número MDL:
MFCD00130719
Código UNSPSC:
12352204
NACRES:
NA.54

forma

lyophilized powder

Nível de qualidade

300

atividade específica

≥3 units/mg protein

peso molecular

homodimer ~90 kDa

composição

Protein, ~70% biuret

solubilidade

H2O: soluble 0.5 mg/mL

temperatura de armazenamento

−20°C

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Aplicação

Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.

Ações bioquímicas/fisiológicas

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.

Definição da unidade

One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

forma física

Contains sodium acetate salt.

Nota de preparo

Chromatographically purified
Solutions should be prepared fresh prior to use.

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palavra indicadora

Danger

Frases de perigo

H315,H319,H334,H335

Classificações de perigo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

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Jeremy P Mallari et al.
Bioorganic & medicinal chemistry, 12(22), 6011-6020 (2004-10-23)
A series of alkyl and aryl phosphonyl, thiophosphonyl, and dithiophosphonyl derivatives of (S)- and (R)-glutamic acid were prepared and examined for inhibitory potency against glutamate carboxypeptidase (carboxypeptidase G). The acquisition of the phosphonamidodithioic acids and the individual phosphonamidothioic acid diastereomers
Takashi Tsukamoto et al.
Bioorganic & medicinal chemistry letters, 12(16), 2189-2192 (2002-07-20)
Phosphonate and phosphinate analogues of N-acylated gamma-glutamylglutamate were tested for the ability to inhibit glutamate carboxypeptidase II (GCP II). All of the compounds inhibit GCP II with IC(50) values in the low nanomolar range. The comparison of the results to
Masaharu Suzuki et al.
Current opinion in plant biology, 11(5), 548-553 (2008-08-12)
Two subfamilies of plant-specific B3 domain transcription factors regulate the fundamental transition between seed and vegetative phases of development. The AFL B3 genes activate the embryo maturation program, while the closely related VAL B3 genes shutdown the AFL network before
Edgar M Pera et al.
Gene expression patterns : GEP, 3(2), 147-152 (2003-04-25)
Endoderm development is an area of intense interest in developmental biology, but progress has been hampered by the lack of specific markers for differentiated endodermal cells. In an unbiased secretion cloning screen of Xenopus gastrula embryos we isolated a novel
The enzymatic hydrolysis of methotrexate and folic acid.
C C Levy et al.
The Journal of biological chemistry, 242(12), 2933-2938 (1967-06-25)
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