C8274
Cytohelicase from Helix pomatia
lyophilized powder
Sinônimo(s):
Snail gut enzyme
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About This Item
Produtos recomendados
forma
lyophilized powder
Nível de qualidade
composição
Protein, 50-80% Lowry
temperatura de armazenamento
2-8°C
Aplicação
Cytohelicase from Helix pomatia has been used:
- in the enzyme solution for preparing the cell nuclei for immuno fluorescent in situ hybridization (immunoFISH)
- in the enzyme mix to analyze meiotic cells at diakinesis and later stages in anthers
- in the enzyme mix for the analysis of mitosis in anthers
Cytohelicase from Helix pomatia has been used to study the isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia.
The enzyme from Sigma has been used for cytohelicase assay. The enzyme assay was used while studying the effects of MNN10 gene-disruption on protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae cultures.
Ações bioquímicas/fisiológicas
Cytohelicase from Helix pomatia is used in the preparation of synaptonemal complex spreading techniques along with bovine serum albumin. It plays an important role in the digestion of cell wall. Cytohelicase interacts with chromatin and improves the adhesion of synaptonemal complexes to the hydrophobic plastic film.
Nota de análise
Contains glucanase/laminarinase activity
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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M Diatewa et al.
Biochimie, 63(1), 67-69 (1981-01-01)
Conversion of large amounts of Saccharomyces cerevisiae cells to protoplasts is studied using various preparations extracted from Helix pomatia hepato-pancreatic juice. The most favourable yield in two hours incubations (88 per cent) is obtained with 20 ml cytohelicase, a chitinase
Pairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii X S. tuberosum (potato) interspecific hybrids.
Gaiero P, et al.
Euphytica, 213(7), 137-137 (2017)
The effect of bovine serum albumin and cytohelicase on surface-spread synaptonemal complexes of Rye (Secale cereale).
De Jong JH and van Eden J
Stain Technology, 63(4), 213-220 (1988)
Improved method of enzyme digestion for root tip cytology.
Lattier JD, et al.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 52(7), 1029-1032 (2017)
ImmunoFISH: simultaneous visualisation of proteins and DNA sequences gives insight into meiotic processes in nuclei of grasses.
Sepsi A, et al.
Frontiers in Plant Science, 9(7), 1193-1193 (2018)
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