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P5936

Sigma-Aldrich

Pectolyase from Aspergillus japonicus

greener alternative

suitable for plant cell culture, lyophilized powder

Sinônimo(s):

PEL1

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About This Item

Número da licença da enzima:
3.2.1.15 (BRENDA, IUBMB)
Número MDL:
MFCD00131813
Código UNSPSC:
10171502
NACRES:
NA.72

forma

lyophilized powder

Nível de qualidade

200

atividade específica

≥0.3 unit/mg solid

características do produto alternativo mais ecológico

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

técnica(s)

cell culture | plant: suitable

aplicação(ões)

agriculture

categoria alternativa mais ecológica

Enabling,

temperatura de armazenamento

2-8°C

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Descrição geral

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Aplicação

Pectolyase P5936 (Pel1) is a natural mixed pectolyase produced by the fungus Aspergillus japonicus used to digest components (endopolygalacturonate and pectin) of plant cell walls. Treatment of cell walls with pectolyase can be used to destablilize the cell walls for organelle isolation or to modify the elasticity of the cell walls.
Pectolyase from Aspergillus japonicas has been used to digest the siliques and also in immunofluorescence experiments.
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Outras notas

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

Definição da unidade

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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AtKP1, a kinesin-like protein, mainly localizes to mitochondria in Arabidopsis thaliana
Ni C Z, et al.
Cell Research, 15(9), 725-725 (2005)
Nuclear fusions contribute to polyploidization of the gigantic nuclei in the chalazal endosperm of Arabidopsis
Baroux C, et al.
Planta, 220(1), 38-46 (2004)
Qingxin Zhao et al.
Plant physiology, 147(4), 1874-1885 (2008-06-20)
Mature plant cell walls lose their ability to expand and become unresponsive to expansin. This phenomenon is believed to be due to cross-linking of hemicellulose, pectin, or phenolic groups in the wall. By screening various hydrolytic enzymes, we found that
Detection of the quantity of kinesin and microgravity-sensitive kinesin genes in rat bone marrow stromal cells grown in a simulated microgravity environment
Ni C, et al.
Acta Astronautica, 68(11-12), 1722-1728 (2011)
Sara Posé et al.
Journal of experimental botany, 64(12), 3803-3815 (2013-07-23)
Antisense-mediated down-regulation of the fruit-specific polygalacturonase (PG) gene FaPG1 in strawberries (Fragaria×ananassa Duch.) has been previously demonstrated to reduce fruit softening and to extend post-harvest shelf life, despite the low PG activity detected in this fruit. The improved fruit traits
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