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P4716

Sigma-Aldrich

Pectinase from Aspergillus niger

greener alternative

BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

Sinônimo(s):

Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger

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About This Item

Número CAS:
9032-75-1
Número da licença da enzima:
3.2.1.15 (BRENDA, IUBMB)
Número CE:
232-885-6
Número MDL:
MFCD00131809
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

Aspergillus niger

Nível de qualidade

200

linha de produto

BioReagent

Formulário

aqueous glycerol solution

atividade específica

≥5 units/mg protein (Lowry)

características do produto alternativo mais ecológico

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

técnica(s)

cell culture | plant: suitable

categoria alternativa mais ecológica

, Enabling

temperatura de armazenamento

2-8°C

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Descrição geral

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Aplicação

Petctinase from Aspergillus niger has been used:
  • for protoplasts liberation
  • in the protoplast enzyme mixture for enzyme digestion
  • to conduct partial saccharification of sugars
  • to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Ações bioquímicas/fisiológicas

Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans.

Definição da unidade

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

forma física

Solution in 40% glycerol

Outras notas

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
SLBT6394
SLBH8994V
SLBG1533V
SLBB1928V
027K06981V

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A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

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Enzymatic Assay of Pectinase

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