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Key Documents

C7082

Sigma-Aldrich

Monoclonal Anti-β-Catenin antibody produced in mouse

clone 6F9, ascites fluid

Sinônimo(s):

Anti-CTNNB, Anti-EVR7, Anti-MRD19, Anti-NEDSDV, Anti-armadillo

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

ascites fluid

tipo de produto de anticorpo

primary antibodies

clone

6F9, monoclonal

peso molecular

antigen 94 kDa

contém

15 mM sodium azide

reatividade de espécies

human, chicken, canine, bovine

técnica(s)

indirect immunofluorescence: 1:1,000 using cultured MDBK cells
microarray: suitable
western blot: 1:1,000 using cultured MDBK cells extract

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... CTNNB1(1499)

Descrição geral

Monoclonal Anti- β -Catenin (mouse IgG1 isotype) is derived from the 6F9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The protein encoded is also called as cadherin-associated protein.The encoded protein β-catenin is localized to the nucleus. Monoclonal antibody reacting specifically with β -catenin is an essential tool in defining the interactions and distributions of β -catenin and its relationships with other catenins and cadherins in various cells and tissues.

Especificidade

The antibody does not cross-react with plakoglobin (γ-catenin).

Imunogênio

recombinant chicken β-catenin.

Aplicação

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-β-Catenin antibody produced in mouse has been used in:
  • immunohistochemistry
  • immunofluorescence
  • immunoblotting

Ações bioquímicas/fisiológicas

In Wnt/β-catenin signal pathway, β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The encoded protein has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression of colon cancer progression. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. Monoclonal antibody reacting specifically with β -catenin is an essential tool in defining the interactions and distributions of β -catenin and its relationships with other catenins and cadherins in various cells and tissues.

forma física

Supplied as ascites fluid with 15 mM sodium azide as a preservative.

Armazenamento e estabilidade

For extended storage freeze in working aliquots. For continuous use, store at 2-8 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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