C2780
Condroitinase AC
lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
Sinônimo(s):
Condroitina AC liase
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About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
Produtos recomendados
conjugado
(Glucosaminoglycan)
Nível de qualidade
Formulário
lyophilized powder
atividade específica
0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
composição
Protein, ~15% Lowry
solubilidade
0.02 M phosphate buffer: soluble (pH 7.0)
atividade externa
Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount
temperatura de armazenamento
−20°C
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Aplicação
Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.
Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
Ações bioquímicas/fisiológicas
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
Outras notas
Contains potassium phosphate buffer salts and BSA as stabilizer.
Veja mais informações sobre enzimas para análise de carboidratos complexos em www.sigma-aldrich.com/enzymeexplorer
Adequação
Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides
Definição da unidade
One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).
Nota de preparo
Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.
Aplicação
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Artigos
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
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