C0543

Rat Collagen Type IV

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

• Sinônimo(s): Type IV collagen, EHS-collagen
• Número CAS: 9007-34-5
• Número CE: 232-697-4
• Número MDL: MFCD00130825
• Código UNSPSC: 12352202
• NACRES: NA.75

Propriedades

• product name: Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
• fonte biológica: mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
• Nível de qualidade: 200
• tipo: Type IV (Miller)
• linha de produto: BioReagent
• forma: lyophilized powder
• embalagem: glass bottle of 1 vial
• técnica(s): cell culture | mammalian: suitable
• cobertura de superfície: 6‑10 μg/cm²
• solubilidade: 0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
• nº de adesão UniProt: B1AVK5; P02463; P08122; Q63ZW6; Q9QZS0
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: mouse ... Col4a1(12826) , Col4a2(12827) , Col4a3(12828) , Col4a5(12830) , Col4a6(94216)

Descrição

Descrição geral

Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Aplicação

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:

• in adhesion assays.
• in hydrogel fabrication.
• to coat cell culture plates for cell differentiation.
• to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.

Recommended for use as a cell culture substratum at 6-10 μg/cm².

Ações bioquímicas/fisiológicas

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Outras notas

Native collagen

We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Quantidade

0.75 mg/vial

Nota de preparo

Chloroform treated.
Pepsin is not used in the preparation.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)