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Key Documents

B3640

Sigma-Aldrich

Anti-Biotin antibody produced in goat

affinity isolated antibody, lyophilized powder

Sinônimo(s):

Anti-Biotin, Anti Biotin Antibody, Anti Biotin Antibody - Anti-Biotin antibody produced in goat, Anti-Biotin Antibody

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

goat

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

lyophilized powder

técnica(s)

immunoelectrophoresis: suitable

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

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Descrição geral

Biotin is considered as an essential nutrient, though it is know to be secreted by some of the intestinal bacteria. It is found in liver and brain tissues in minute quantities.
Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.
Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.

Imunogênio

Biotin-KLH conjugate

Aplicação

Anti-Biotin antibody produced in goat has been used in multi-well plate assay, to identify desthiobiotinylating (analog of biotin) proteins. It has also been used in Colourimetric enzyme immunoassay.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Ações bioquímicas/fisiológicas

Biotin serves as a cofactor for carboxylases, necessary for oxidative metabolism. Biotin absorption in small intestine is reduced on consumption of avidin rich food such as egg white. Thus, greatly decreasing its bioavailability, leading to egg white injury. Reduced levels of biotin is normally observed during pregnancy. Biotin deficiency might be congenital, with defective proteins involved in biotin homeostasis.

forma física

Lyophilized from 0.01 M sodium phosphate, 15 mM sodium chloride, pH 7.2

Reconstituição

Reconstitute with 0.135 M sodium chloride.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Marcus Braun et al.
Nature cell biology, 13(10), 1259-1264 (2011-09-06)
Short regions of overlap between ends of antiparallel microtubules are central elements within bipolar microtubule arrays. Although their formation requires motors, recent in vitro studies demonstrated that stable overlaps cannot be generated by molecular motors alone. Motors either slide microtubules
Zdenek Lansky et al.
Cell, 160(6), 1159-1168 (2015-03-10)
Cytoskeletal remodeling is essential to eukaryotic cell division and morphogenesis. The mechanical forces driving the restructuring are attributed to the action of molecular motors and the dynamics of cytoskeletal filaments, which both consume chemical energy. By contrast, non-enzymatic filament crosslinkers
A R Prescott et al.
Journal of cell science, 102 ( Pt 1), 103-112 (1992-05-01)
The turnover of microtubules was studied in the processes of PtK2 cells, after treatment with the cytokine scatter factor (SF), using micro-injected biotin-tubulin as a reporter of new microtubule growth. Cells treated with SF became dispersed and fibroblast-like in morphology
Vito Cataldo et al.
Electroanalysis, 20(2), 115-122 (2008-10-11)
Amperometric immunosensor configurations featuring covalently bound anti-biotin antibodies (Ab) embedded into a polylysine (PLL)-single walled carbon nanotube (SWCNT) composite layer were evaluated. Assemblies were made by first oxidizing pyrolytic graphite (PG) electrodes to form surface carboxylic acid groups, to which
Avidin-Biotin Interactions: Methods and Applications, 111-111 (2008)

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