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A8011

Sigma-Aldrich

Antifoam C Emulsion

aqueous-silicone emulsion

Sinônimo(s):

Antifoaming Agent Emulsion

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About This Item

Código UNSPSC:
12161900
NACRES:
NA.25

fonte biológica

synthetic

forma

emulsion

contém

emulsifier

técnica(s)

cell culture | hybridoma: suitable
microbiological culture: suitable

pf

0  °C (32 °F)

Descrição geral

Antifoam C emulsion is an aqueous emulsion containing 30% active silicone. This silicone-based antifoam emulsion consists of non-ionic emulsifiers different from those in antifoam emulsions B and Y-30. Antifoam C emulsion can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately. Antifoam C emulsion is typically effective at 1-10 ppm. The flow properties of antifoam C emulsion are such that it can be pumped on an as-needed basis to a fermenter system with sufficient agitation to disperse the antifoam. Antifoam C emulsion can be used to regulate headspace foaming in mammalian cell culture bioreactors up to 30 ppm concentration.

Aplicação

Antifoam C emulsion has been used:
  • as a supplement in RO-H2O and a chemically defined culture medium to determine volumetric mass transfer coefficient (kLa) of mammalian cell culture
  • as a component of feed medium,
  • as a steam-sterilized antifoaming agent in a biocontroller for pH-stat fed-batch cultivations
  • as a supplement in fed batch medium to mimic the typical large-scale cultivation conditions

Características e benefícios

  • Minimizes compatibility issues with biological systems
  • Highly effective defoamer at low concentrations
  • Highly effective and versatile silicone defoamer

Outras notas

A 30% aqueous emulsion of polydimethylsiloxane concentrate.
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Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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This study demonstrates the feasibility of large-scale production of murine polyomavirus VP1 protein in recombinant Escherichia coli as pentamers which are able to subsequently self-assemble in vitro into virus-like particles (VLPs). High-cell-density pH-stat fed-batch cultivation was employed to produce glutathione-S-transferase
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